Each card follows the same sequence, but the concentration changes when either the total peptide amount or the recorded water volume changes. The first two cards keep the total at 5 mg and change only the water volume. Moving from 2 mL to 1 mL doubles the concentration from 2.5 mg/mL to 5 mg/mL, so the same 250 mcg amount becomes half as much liquid and half as many U-100 units.
The second and third cards share a 5 mg/mL concentration even though their vial totals and water volumes differ. Their entered amounts then lead to different liquid volumes, while both happen to land at 10 units. This is why the vial label and recorded liquid volume belong together: neither number alone tells you the U-100 mark.
The calculation starts with the exact numbers for the vial you are reading, carries units through each step, and finishes with a scale mark. Keeping each unit visible makes the calculation easier to review, compare, and check against the vial label. The figures in these cards are arithmetic examples, not defaults for a vial or instructions for use.